Journal: Molecules

Article Title: New Glycotoxin Inhibitor from Sesuvium sesuvioides Mitigates Symptoms of Insulin Resistance and Diabetes by Suppressing AGE-RAGE Axis in Skeletal Muscle

doi: 10.3390/molecules29153649

Figure Lengend Snippet: Effect of compound 1 on symptoms of diabetes in a mouse model of diabetes. ( A ) Glucose tolerance. ( B ) Insulin levels. ( C ) Insulin levels. ( D ) Adiponectin levels. ( E ) Insulin resistance. ( F ) Glycogen synthesis in muscles. Periodic acid–Schiff staining of gastrocnemius muscle. Results represent mean ± SEMs. n = 10–20 in each group; * p < 0.05, diabetic mice vs. treated with 1 .

Article Snippet: Cellular and serum AGEs, insulin, and adiponectin levels were measured by the quantitative sandwich enzyme immunoassay technique by using an enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer’s instructions (STA-817, CML kit, San Diego, CA, USA), Mice insulin ELISA kit, and Mice adiponectin ELISA kit (DRP-300, R&D System, Minneapolis, MN, USA).

Techniques: Muscles, Staining

Journal: Journal of Biological Engineering

Article Title: Sustainable production of multimeric and functional recombinant human adiponectin using genome-edited chickens

doi: 10.1186/s13036-024-00427-2

Figure Lengend Snippet: Sustainability of multimeric patterns in recombinant human adiponectin (hADPN) across subsequent generations of ADPN knock-in (KI) genome-edited chickens ( A ) Pedigree of ovalbumin ( OVA ) ADPN KI genome-edited chickens across generations. ( B ) Quantitative analysis of total ADPN protein abundance in genome-edited hens across generations. ( C ) Quantitative analysis of high molecular weight (HMW) ADPN protein abundance of genome-edited hens across generations. Statistical analysis was performed using one-way ANOVA ( n = 3). Significant differences are indicated as “ns”, no significance. ( D , E ) Western blotting analysis of ADPN in OVA ADPN KI chicken egg white under non-reducing and reducing conditions. Arrows indicate the forms of ADPN, including HMW, hexamer (medium), trimer (low), and monomer

Article Snippet: The quantities of total ADPN and HMW ADPN from OVA ADPN KI EW were measured using human total ADPN quantikine ELISA kit (DRP300; R&D systems, Minneapolis, MN, USA) and human HMW ADPN quantikine ELISA kit (DHWAD0; R&D systems) respectively.

Techniques: Recombinant, Knock-In, Quantitative Proteomics, High Molecular Weight, Western Blot

Journal: Journal of Biological Engineering

Article Title: Sustainable production of multimeric and functional recombinant human adiponectin using genome-edited chickens

doi: 10.1186/s13036-024-00427-2

Figure Lengend Snippet: Comparative analysis of the multimeric composition of recombinant hADPN ( A ) Detection of multimeric recombinant hADPN derived from OVA ADPN KI chicken egg white (EW)-derived hADPN and other commercial recombinant hADPNs derived from human embryonic kidney (HEK) 293 cells with a C-terminal FLAG tag, High-Five (Hi-5) cells, and human serum (positive control) by western blotting analysis under non-reducing condition. ( B ) Detection of reduced EW-derived recombinant hADPN and other commercial recombinant hADPNs derived from HEK293 cells, Hi-5 cells, and human serum by western blotting analysis. Arrows indicate HMW, hexamer (medium), trimer (low), and monomer forms of hADPN.

Article Snippet: The quantities of total ADPN and HMW ADPN from OVA ADPN KI EW were measured using human total ADPN quantikine ELISA kit (DRP300; R&D systems, Minneapolis, MN, USA) and human HMW ADPN quantikine ELISA kit (DHWAD0; R&D systems) respectively.

Techniques: Recombinant, Derivative Assay, FLAG-tag, Positive Control, Western Blot

Journal: Journal of Biological Engineering

Article Title: Sustainable production of multimeric and functional recombinant human adiponectin using genome-edited chickens

doi: 10.1186/s13036-024-00427-2

Figure Lengend Snippet: Comparative analysis of endoplasmic reticulum (ER) chaperone gene transcription in the adipose tissue, oviduct magnum of wild-type (WT) and ADPN KI hens, and HEK293 cells ( A − D ) mRNA expression levels of Ero1-Lα , DsbA-L , ERP44 , and PDI in the adipose tissue and oviduct magnum of WT and ADPN KI hens compared with HEK293 cells. Samples from WT hens are denoted as WT adipose tissue and WT oviduct magnum, while samples from ADPN KI hens are labeled as ADPN adipose tissue and ADPN oviduct magnum. Statistical analysis was performed using one-way ANOVA ( n = 3). Different superscript letters indicate significant differences between groups ( p < 0.05)

Article Snippet: The quantities of total ADPN and HMW ADPN from OVA ADPN KI EW were measured using human total ADPN quantikine ELISA kit (DRP300; R&D systems, Minneapolis, MN, USA) and human HMW ADPN quantikine ELISA kit (DHWAD0; R&D systems) respectively.

Techniques: Expressing, Labeling